LAMP1 (3C3) Monoclonal Antibody

$Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with LAMP1 (3C3) Monoclonal Antibody (bsm-51301M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).$

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with LAMP1 (3C3) Monoclonal Antibody (bsm-51301M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).

$Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with LAMP1 (3C3) Monoclonal Antibody (bsm-51301M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with LAMP1 (3C3) Monoclonal Antibody (bsm-51301M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$ane 1: Human HT1080 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U937 cell lysates probed with LAMP1 Monoclonal Antibody, Unconjugated (bsm-51301M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

ane 1: Human HT1080 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U937 cell lysates probed with LAMP1 Monoclonal Antibody, Unconjugated (bsm-51301M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF

Reactivity

Human

Overview
Catalog #	bsm-51301M
Product Name	LAMP1 (3C3) Monoclonal Antibody
Applications	WB, IHC-P, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This LAMP1 antibody is generated from a mouse immunized with a recombinant protein.
Clonality	Monoclonal
Clone #	3C3
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3916
Swiss Prot	P11279
Synonyms	LAMPA; CD107a; LGP120; Lysosome-associated membrane glycoprotein 1; LAMP-1; Lysosome-associated membrane protein 1; CD107 antigen-like family member A; LAMP1
Background	Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. Acts as a receptor for Lassa virus protein.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF