New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

ATG3 (7C2) Monoclonal Antibody

Applications

  • WB
  • IHC-P

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-51284M
Product Name ATG3 (7C2) Monoclonal Antibody
Applications WB, IHC-P
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Mouse
Source This ATG3 antibody is generated from a mouse immunized with recombinant protein of human ATG3.
Clonality Monoclonal
Clone # 7C2
Isotype IgG1
Concentration 0.5ug/ul
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 64422
Swiss Prot Q9NT62
Synonyms APG3; APG3L; PC3-96; APG3-LIKE; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; hApg3; Protein PC3-96; ATG3
Background E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400