MSH2 (4C2) Monoclonal Antibody

ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.", "Category"=>"Primary Antibody", "Clonality"=>"Monoclonal", "Clone Number"=>"4C2", "Concentration"=>"0.5ug/ul", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bsm-51251M", "Gene ID"=>"4436", "Gene ID Link"=>"Entrez Gene: 4436", "Host"=>"Mouse", "images"=>"[{\"id\":\"44372\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"5\",\"origin_notes\":\"Abcepta\",\"product_id\":\"13031\",\"productconjugation_id\":null,\"application_id\":\"1\",\"application_note\":\"\",\"dilution\":\"1:2000\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Lane 1: 293 Cell lysates; Lane 2: HeLa Cell lysates; probed with MSH2 (1184CT1.3.2) Monoclonal Antibody, unconjugated (bsm-51251M) at 1:1000 overnight at 4\\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\\u00b0C.\",\"sample_type_id\":\"1\",\"sample_details\":null,\"rating\":\"2\",\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":\"13\",\"protocols_used\":\"MSH2 Antibody (Center) (Cat. #AM2242b) western blot analysis in 293,Hela cell line lysates (35\\u03bcg\\/lane).This demonstrates the MSH2 antibody detected the MSH2 protein (arrow).\",\"additional_notes\":\"\",\"filepath_raw\":\"13031\\/44372_raw.png\",\"filepath_med\":\"13031\\/44372_med.jpg\",\"filepath_thumb\":\"13031\\/44372_thumb.jpg\",\"display_order\":\"2\",\"lock_version\":\"1\",\"created_at\":\"2023-06-02 19:10:11\",\"updated_at\":\"2023-06-02 19:10:11\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/44372\"}]", "Isotype"=>"IgG1", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Notes"=>"__;__", "Product URL"=>"https://www.biossusa.com/products/bsm-51251M", "Purification"=>"Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.", "Size"=>"100ul", "Source"=>"Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.", "Storage"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Store at -20°C for 12 months.", "Storage Buffer"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.", "Storage Condition"=>"Store at -20°C for 12 months.", "Swiss Prot"=>"P43246", "Swiss Prot Link"=>"Swiss Prot: P43246", "Synonyms"=>"FCC1; COCA1; HNPCC; LCFS2; HNPCC1; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2", "Target Protein"=>"MSH2"} -->

$Lane 1: 293 Cell lysates; Lane 2: HeLa Cell lysates; probed with MSH2 (1184CT1.3.2) Monoclonal Antibody, unconjugated (bsm-51251M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293 Cell lysates; Lane 2: HeLa Cell lysates; probed with MSH2 (1184CT1.3.2) Monoclonal Antibody, unconjugated (bsm-51251M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-51251M
Product Name	MSH2 (4C2) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	4C2
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4436
Swiss Prot	P43246
Synonyms	FCC1; COCA1; HNPCC; LCFS2; HNPCC1; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2
Background	Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Application Dilution
WB	1:300-5000
IHC-P	1:20-100