TFRC (4C1) Monoclonal Antibody

$Lane 1: HT-1080; Lane 2: HeLa lysate at 20 \u00b5g per lane, probed with bsm-51161M TFRC (514CT23.4.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: HT-1080; Lane 2: HeLa lysate at 20 \u00b5g per lane, probed with bsm-51161M TFRC (514CT23.4.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51161M
Product Name	TFRC (4C1) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This TFRC antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from human TFRC.
Clonality	Monoclonal
Clone #	4C1
Isotype	IgM
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7037
Swiss Prot	P02786
Synonyms	T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46; Transferrin receptor protein 1; TFRC
Background	Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Application Dilution
WB	1:300-5000