HGF (9B6) Monoclonal Antibody

$Lane 1: HepG2; Lane 2: Ramos lysate at 20 \u00b5g per lane, probed with bsm-51153M HGF (489CT6.12.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: HepG2; Lane 2: Ramos lysate at 20 \u00b5g per lane, probed with bsm-51153M HGF (489CT6.12.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51153M
Product Name	HGF (9B6) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This HGF antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 521-554 amino acids from human HGF.
Immunogen Range	521-554/723
Clonality	Monoclonal
Clone #	9B6
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3082
Swiss Prot	P14210
Synonyms	SF; HGFB; HPTA; F-TCF; DFNB39; Hepatocyte growth factor; Hepatopoietin-A; Scatter factor; HGF
Background	Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
Application Dilution
WB	1:300-5000