BMI1 (2T6) Monoclonal Antibody

$Lane 1: HeLa; Lane 2: A549 lysate at 20 \u00b5g per lane, probed with bsm-51082M BMI1 (282CT3.7.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: HeLa; Lane 2: A549 lysate at 20 \u00b5g per lane, probed with bsm-51082M BMI1 (282CT3.7.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51082M
Product Name	BMI1 (2T6) Monoclonal Antibody
Applications	WB, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein.
Clonality	Monoclonal
Clone #	2T6
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	100532731, 648
Swiss Prot	P35226
Background	Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Application Dilution
WB	1:300-5000
IF