TBB1 (6A4) Monoclonal Antibody

$Lane 1: CCRF-CEM cell lysate (20ug); Lane 2: HeLa cell lysate (20ug) probed with bsm-51009M TBB1 (6A4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: CCRF-CEM cell lysate (20ug); Lane 2: HeLa cell lysate (20ug) probed with bsm-51009M TBB1 (6A4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

$Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBB1 (6A4) Monoclonal Antibody, Unconjugated (bs-51009R) at 1:10 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBB1 (6A4) Monoclonal Antibody, Unconjugated (bs-51009R) at 1:10 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

HepG2 cells stained with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:25 dilution, followed by Alexa Fluor 488-conjugated secondary antibody (green), nuclear staining (blue).

Applications

WB
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51009M
Product Name	TBB1 (6A4) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This monoclonal antibody is generated from mice immunized with a recombinant protein human TBB1.
Clonality	Monoclonal
Clone #	6A4
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	81027
Swiss Prot	Q9H4B7
Synonyms	Tubulin beta-1 chain; TUBB1
Background	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400