VEGF-A (5F4) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with VEGF-A (5F4) Monoclonal Antibody, Unconjugated (bsm-4572M) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with VEGF-A (5F4) Monoclonal Antibody, Unconjugated (bsm-4572M) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

FCM
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Rat
Guinea Pig

Predicted Reactivity

Mouse
Dog
Cow
Sheep
Pig
Chicken

Overview
Catalog #	bsm-4572M
Product Name	VEGF-A (5F4) Monoclonal Antibody
Applications	FCM, IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human, Rat, Guinea Pig
Predicted Reactivity	Mouse, Dog, Cow, Sheep, Pig, Chicken
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human VEGF-A
Clonality	Monoclonal
Clone #	5F4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition	Store at -20¡C for 12 months.
Target
Gene ID	7422
Swiss Prot	P15692
Synonyms	Vascuoar endothelial growth factor A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGF164; VEGFA; MGC70609; MVCD1; VEGF; VPF.
Background	This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq].
Application Dilution
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF