Renin Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Renin) Monoclonal Antibody, Unconjugated (bsm-43146M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Renin) Monoclonal Antibody, Unconjugated (bsm-43146M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Lane 1: Recombinant human Renin protein, C-His (HEK293)(bs-43146P) probed with Renin Monoclonal Antibody, Unconjugated (bsm-43146M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant human Renin protein, C-His (HEK293)(bs-43146P) probed with Renin Monoclonal Antibody, Unconjugated (bsm-43146M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

IHC-P

Reactivity

Human

Overview
Catalog #	bsm-43146M
Product Name	Renin Monoclonal Antibody
Applications	IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human Renin protein
Clonality	Monoclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	PBS (pH=7.4)
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5972
Swiss Prot	P00797
Subcellular location	Secreted, Cell membrane
Synonyms	Angiotensin forming enzyme; Angiotensin forming enzyme precursor; Angiotensinogenase; Angiotensinogenase precursor; HNFJ2; REN; Renin1; Renin-1; Renin 1; Ren1; RENI_HUMAN; Renin; Renin precursor renal.
Application Dilution
IHC-P	=1:100-500