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p57 Kip2 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (p57 Kip2 ) Monoclonal Antibody, Unconjugated (bsm-34156M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (p57 Kip2 ) Monoclonal Antibody, Unconjugated (bsm-34156M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bsm-34156m-100ul
Product Name p57 Kip2 Monoclonal Antibody
Applications IHC-P, IHC-F, IF
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human p57 Kip2
Clonality Monoclonal
Clone # 4.00E+08
Isotype IgG2b/Kappa
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1028
Swiss Prot P49918
Subcellular location Nucleus
Synonyms Beckwith Wiedemann syndrome; BWCR; BWS; CDKI; CDKN 1C; CDKN1C; Cyclin dependent kinase inhibitor 1C; Cyclin dependent kinase inhibitor p57; KIP 2; KIP2; p57; p57 Kip2; p57 Kip 2; p57Kip2; WBS; IMAGE; CDN1C_HUMAN.
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500

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