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MLH1 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MLH1) Monoclonal Antibody, Unconjugated (bsm-34145M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MLH1) Monoclonal Antibody, Unconjugated (bsm-34145M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-34145m-100ul
Product Name MLH1 Monoclonal Antibody
Applications IHC-P, IHC-F, IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human MLH1
Clonality Monoclonal
Clone # 4A13
Isotype IgG1/Kappa
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4292
Swiss Prot P40692
Subcellular location Nucleus
Synonyms MLH1_HUMAN; DNA mismatch repair protein Mlh1; COCA2; MutL protein homolog 1; FCC2; HNPCC; MLH-1; hMLH1; HNPCC2; MMRCS1;
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:50-200

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