Cytokeratin 20 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (bsm-34143M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (bsm-34143M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (bsm-34143M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Cytokeratin 20) Monoclonal Antibody, Unconjugated (bsm-34143M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

IHC-P

Reactivity

Human

Overview
Catalog #	bsm-34143M
Product Name	Cytokeratin 20 Monoclonal Antibody
Applications	IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human CK20
Clonality	Monoclonal
Clone #	1F9
Isotype	IgG2a/Kappa
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.136% sodium azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	54474
Swiss Prot	P35900
Subcellular location	Cytoplasm
Synonyms	CK 20; CK20; Cytokeratin20; K20; Keratin 20; Keratin type I cytoskeletal 20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; Protein IT; K1C20_HUMAN.
Application Dilution
IHC-P	=1:100-500