AMPK b1 Monoclonal Antibody

$Lane 1: K562 cell lysates; Lane 2: Molt-4 cell lysates; Lane 3: HepG2 cell lysates probed with AMPK b1 Monoclonal Antibody, Unconjugated (bsm-33427M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

Lane 1: K562 cell lysates; Lane 2: Molt-4 cell lysates; Lane 3: HepG2 cell lysates probed with AMPK b1 Monoclonal Antibody, Unconjugated (bsm-33427M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-33427M
Product Name	AMPK b1 Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human AMPK b1 Protein
Clonality	Monoclonal
Clone #	2F2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	Q9Y478
Swiss Prot	5564
Synonyms	5 AMP activated protein kinase subunit beta 1; AMPK; AMPK beta 1 chain; AMPKb; HAMPKb; PRKAB1; 5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK beta -1 chain; 5'-AMP-activated protein kinase beta-1 subunit; AMPKb; AMPK subunit beta-1; AAKB1_HUMAN; AMPK b1; AMPK-b1.
Background	The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:300-5000