AFP (A2) Monoclonal Antibody

Lane 1: Rat Brain lysates; Lane 2: Rat Kidney lysates; Probed with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200, overnight at 4˚C. Followed secondary antibody (bs-0296G-HRP) at 1:3000 for 90min at 37˚C.

$Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.

$Lane 1: Rat Embryo Lysates; Lane 2: Rat Placenta Lysates. Probed with AFP(A2) monoclonal Antibody, unconjugated (bsm-1621M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Embryo Lysates; Lane 2: Rat Placenta Lysates. Probed with AFP(A2) monoclonal Antibody, unconjugated (bsm-1621M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IF(IHC-P)

Reactivity

Human
Rat

Overview
Catalog #	bsm-1621M
Product Name	AFP (A2) Monoclonal Antibody
Applications	WB, ELISA, IHC-P, IF(IHC-P)
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Full length human AFP protein
Clonality	Monoclonal
Clone #	A2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	174
Swiss Prot	P02771
Synonyms	AFPD; FETA; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; AFP
Background	Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200