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AFP (A2) Monoclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P

Reactivity

  • Human
  • Rat
Overview
Catalog # bsm-1621M-HRP
Product Name AFP (A2) Monoclonal Antibody, HRP Conjugated
Applications WB, ELISA, IHC-P
Reactivity Human, Rat
Specifications
Conjugation HRP
Host Mouse
Source Full length human AFP protein
Clonality Monoclonal
Clone # A2
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 174
Swiss Prot P02771
Synonyms AFPD; FETA; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; AFP
Background Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400