BCL-2 (1A5) Monoclonal Antibody

$Bcl-2 protein lysates probed with BCL-2 (1A5) Monoclonal Antibody bsm-10846M at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Bcl-2 protein lysates probed with BCL-2 (1A5) Monoclonal Antibody bsm-10846M at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-10846M
Product Name	BCL-2 (1A5) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant protein fragment (1-220 amino acids range) of human Bcl-2.
Clonality	Monoclonal
Clone #	1A5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	596
Swiss Prot	P10415
Synonyms	Apoptosis regulator Bcl-2; BCL2
Background	Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release
Application Dilution
WB	1:300-5000