Human 7-Dehydrocholesterol Reductase (DHCR7) ELISA Kit
Due to the possibility of mismatching between antigens from other origin and antibodies used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.
Principle of the Assay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DHCR7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DHCR7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain DHCR7, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHCR7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures.
Target Information
7-dehydrocholesterol reductase of the cholesterol biosynthetic pathway reducing the C7-C8 double bond of cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol, two intermediates in that pathway.
| GENE ID | 1717 |
| SWISS PROT | Q9UBM7 |
| SYNONYMS |
DHCR-7; SLOS; D7SR; Smith-Lemli-Opitz Syndrome; Putative sterol reductase SR-2; Sterol Delta(7)-reductase |
Materials Supplied
| Kit Components | 96 Wells Quantity/Size |
|---|---|
| Pre-coated, ready-to-use 96-well strip plate | 1 plate |
| Plate sealer for 96 wells | 2 |
| Standard |
2 tubes |
| Diluent buffer | 1 bottle |
| Detection Reagent A | 1 bottle |
| Detection Reagent B | 1 bottle |
| TMB Substrate | 1 tube |
| Stop Solution | 1 tube |
| Wash Buffer (30 ℅ concentrate) | 1 tube |
| Product data sheet | 1 copy |
Storage
| Storage | The TMB Substrate, Wash Buffer (30X concentrate), and the Stop Solution should be stored at 4°C upon receipt, while the other items should be stored at -20°C. |
Performance Characteristics
| REPEATABILITY |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high-level DHCR7 were tested 20 times on one plate, respectively. |
| SENSITIVITY | The minimum detectable dose was 0.062ng/mL. |
| ASSAY RANGE | 0.156-10ng/mL |
| SPECIFICITY | This assay has high sensitivity and excellent specificity for the detection of DHCR7. No significant cross-reactivity or interference between DHCR7 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between DHCR7 and all analogs, therefore, cross reactivity may still exist. |
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