Phospholipase D2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospholipase D2 Polyclonal Antibody, Unconjugated (bs-8747R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospholipase D2 Polyclonal Antibody, Unconjugated (bs-8747R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat
Bovine
Dog
Sheep
Rabbit
Horse

Overview
Catalog #	bs-8747R
Product Name	Phospholipase D2 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PLD2/Phospholipase D2
Immunogen Range	21-120/933
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5338
Swiss Prot	O14939
Subcellular location	Cytomembrane
Synonyms	Choline phosphatase 2; EC 3.1.4.4; hPLD 2; hPLD2; mPLD2; Phosphatidylcholine hydrolyzing phospholipase D2; Phosphatidylcholine-hydrolyzing phospholipase D2; Phospholipase D2; PhospholipaseD2; PLD 2; PLD1C; Pld2; PLD2_HUMAN; Pldc; rPLD2.
Background	May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200