MEPE Polyclonal Antibody

Mouse uterus lysates probed with MEPE Polyclonal Antibody, Unconjugated (bs-8689R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Mouse Cerebellum tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human MCF-7 cell lysates probed with MEPE Polyclonal Antibody, Unconjugated (bs-8689R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Mouse Cerebellum tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human MCF-7 cell lysates probed with MEPE Polyclonal Antibody, Unconjugated (bs-8689R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse

Predicted Reactivity

Human

Overview
Catalog #	bs-8689R
Product Name	MEPE Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MEPE
Immunogen Range	201-300/525
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	56955
Subcellular location	Secreted
Synonyms	Matrix extracellular phosphoglycoprotein; MEPE; MEPE_HUMAN; OF45; Osteoblast/osteocyte factor 45.
Background	MEPE is a 525 amino acid extracellular matrix protein. Expressed in osteocytes and brain, MEPE is a regulator of bone metabolism that is thought to mediate mineralization and demineralization within the osteocyte microenvironment. MEPE contains an RGD cell-attachment motif and shares molecular similarities with several dentin-bone extracellular matrix RGD-containing phosphoglycoproteins, including OPN (osteopontin) and DSP (dentin sialophosphoprotein). Via its ability to control bone mineralization, MEPE is associated with various developmental events such as skeletogenesis, bone regeneration and odontogenesis. MEPE is secreted in hypophosphatemic osteomalacia tumors, suggesting a possible role in the pathophysiology of bone-related cancers.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500