7-Azido-4-methylcoumarin

Overview
Catalog #	bs-75450c-1mg
Product Name	7-Azido-4-methylcoumarin
Specifications
Storage Buffer	Solid
Storage Condition	Stable for 2 years after receipt when stored at -20°C. Store solutions at -20°C in the dark.
Target
Product Information	CAS Number: 95633-27-5 Molecular formula: C10H7N3O2 Molecular weight: 201.2 Purity: >98% (NMR) Appearance: White to yellow solid. Solubility: Soluble in DMSO. InChiKey: HEKDKVLIMUWRRZ-UHFFFAOYSA-N SMILES: CC1=CC(=O)OC2=CC(=CC=C12)N=[N+]=[N-]
Description	Highly sensitive and selective fluorogenic H2S probe [1-3]. The aromatic azide moiety of AzMC is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with lambdaex = 365 nm and lambdaem = 450 nm. Photoaffinity labeling probe for the substrate binding site of human sulfotransferase 1A1 (SULT1A1). Probe to monitor the enzymatic production of H2S in vitro and to visualize H2S in living cells [2, 3]. Tool for monitoring the activity of pyridoxal-5'-phosphate (PLP)-dependent enzymes (e.g. cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CGL) and tryptophan synthase (TS)) [2]. Tool to identify novel cystathionine beta-synthase (CBS) inhibitors and activators [2]. Suitable for high-throughput screening [2]. Caution: Use of this product with DTT, TCEP and/or biological thiols at concentrations of >25 mM should be avoided for maximum efficiency [2].