PGC1 alpha Polyclonal Antibody

Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-PGC1 alpha Polyclonal Antibody, Unconjugated (bs-7535R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 pig muscles lysates L2 mouse kidney lysates probed with Anti PGC1 alpha Polyclonal Antibody, Unconjugated (bs-7535R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 91-110kD. Observed band size: 92kD.

Lane 1: Human MOLT-4 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human HL-60 cell lysates probed with PGC1 alpha + beta Polyclonal Antibody, Unconjugated (bs-7535R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat
Pig

Predicted Reactivity

Mouse
Dog
Cow
Horse
Rabbit

Overview
Catalog #	bs-7535R
Product Name	PGC1 alpha Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Rat, Pig
Predicted Reactivity	Mouse, Dog, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PGC1 alpha
Immunogen Range	151-250/798
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10891
Swiss Prot	Q9UBK2
Subcellular location	Nucleus
Synonyms	LEM6; Ligand effect modulator 6; PERC; Peroxisome prolerative activated receptor, gamma, coactivator 1 alpha; Peroxisome prolerative activated receptor, gamma, coactivator 1; Peroxisome prolerator activated receptor gamma coactivator 1 alpha; peroxisome prolerator-activated receptor gamma coactivator 1 beta; Peroxisome prolerator-activated receptor gamma coactivator 1-alpha; Peroxisome prolerator-activated receptor gamma coactivator 1-beta; peroxisome prolerator-activated receptor gamma, coactivator 1 beta; PGC 1 alpha; PGC 1 alpha; PGC 1v; PGC-1beta; PGC-1-alpha; PGC-1-beta; PGC-1-related estrogen receptor alpha coactivator; PGC1; PGC1alpha; PGC1A; PGC1v; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1beta; PPAR-gamma coactivator 1-alpha; PPAR-gamma coactivator 1-beta; PPARGC 1 alpha; PPARGC-1-alpha; PPARGC-1-beta; PPARGC1; PPARGC1A; Ppargc1b; PRGC1_HUMAN; PRGC2_HUMAN.
Background	The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq].
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200