Boc-Val-Pro-Arg-AMC . HCl

Overview
Catalog #	bs-75321c-5mg
Product Name	Boc-Val-Pro-Arg-AMC . HCl
Specifications
Storage Buffer	Powder
Storage Condition	Stable for 1 year after receipt when stored at -20°C.
Target
Product Information	CAS Number: 65147-04-8 Molecular formula: C31H45N7O7 . HCl Molecular weight: 627.7 . 36.5 Purity: >98% Appearance: Solid lyophilized powder. Solubility: Soluble in water (10mg/ml). Soluble in 5% acetic acid. InChiKey: ROQGJTZGLVLVIN-UHFFFAOYSA-N SMILES: O=C1C=C(C)C2=CC=C(NC(C(CCCNC(N)=N)NC(C3N(C(C(NC(OC(C)(C)C)=O)C(C)C)=O)CCC3)=O)=O)C=C2O1.Cl
Description	Boc-Val-Pro-Arg-AMC is a sensitive fluorogenic peptide substrate for α-thrombin (Km = 21 μM, kcat = 109 s-1) and the α-thrombin-staphylocoagulase complex (Km = 25 μM, kcat = 89 s-1). It also serves as a substrate vor Kallikrein 5 and 8. Activity is quantified by the release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380nm and emits at 440-460nm when interacting with a target enzyme. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).