Semaphorin-6A (cytoplasmic region) Antibody

Applications

Reactivity

Human
Mouse
Rat
Chicken
Xenopus

Overview
Catalog #	bs-70656r-100ul
Product Name	Semaphorin-6A (cytoplasmic region) Antibody
Applications	WB, IP
Specificity	The antibody detects a 120kDa* protein corresponding to the apparent molecular mass of Sema-6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (a.a. 772-787) antibody. The sequence used has high homology to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members.
Reactivity	Human, Mouse, Rat, Chicken, Xenopus
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the cytoplasmic region of human Sema-6A.
Clonality	Polyclonal
Isotype	IgG
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot	Q9H2E6
Background	The Semaphorin family of axon guidance molecules includes secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 6A (Sema-6A) is a single-pass type I membrane protein that contains a plexin-semaphorin-integrin (PSI) domain and a Sema domain. Sema-6A is active as a homodimer or oligomer. The Sema-6A homodimer interacts with a Plexin A2 homodimer. Sema-6a promotes reorganization of the actin cytoskeleton to regulate normal granule cell migration in the developing cerebellum and to control axon guidance in the developing central nervous system. In addition, Sema-6a has been implicated in oligodendrocyte myelination and may alter VEGF signaling during angiogenesis.
Application Dilution
WB	1:300-5000
IP	1-2ug