| Overview |
| bs-70609r-100ul |
| Phosphotyrosine Antibody |
| WB, IP |
| This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells. All peptide sequences used are highly conserved phosphotyrosine motifs. |
| Human, Mouse, Rat, Chicken |
| Specifications |
| Unconjugated |
| Rabbit |
| Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. |
| p-Tyr |
| Polyclonal |
| IgG |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| N/A |
| Phosphotyrosine mAb |
| Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity. |
| Application Dilution |
| WB |
1:300-5000 |
| IP |
1-2ug |
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