eEF2K (Thr-348), Phosphospecific Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-70468r-100ul
Product Name	eEF2K (Thr-348), Phosphospecific Antibody
Applications	WB
Specificity	The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca2+ and Calmodulin. This reactivity is removed after lambda phosphatase treatment. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Phospho-eEF2K (Thr-348) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Thr-348 in human eEF2K.
Modification Site	Thr-348
Clonality	Polyclonal
Isotype	IgG
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot	O00418
Synonyms	eEF-2, eEF-2K, CaMK-III, eukaryotic elongation factor
Background	Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 is an autophosphorylation site that is required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38δ causes inactivation of eEF2K. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.
Application Dilution
WB	1:300-5000