DISC1 (Ser-710), Phosphospecific Antibody

Applications

Reactivity

Mouse
Rat

Overview
Catalog #	bs-70452r-100ul
Product Name	DISC1 (Ser-710), Phosphospecific Antibody
Applications	WB
Specificity	The antibody detects a 100 kDa* protein corresponding to the molecular mass of DISC1 on SDS-PAGE immunoblots of PC12 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. This peptide sequence is well conserved in rat DISC1 (Ser-703), but has five amino acid differences with the conserved site in human DISC1 (Ser-713).
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Phospho-DISC1 (Ser-710) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-710 in mouse DISC1.
Modification Site	Ser-710
Clonality	Polyclonal
Isotype	IgG
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot	Q811T9
Synonyms	disrupted schizophrenia
Background	Disrupted in schizophrenia 1 (DISC1) is a multifunctional scaffold protein that has important roles in neurodevelopment. DISC1 is expressed in both neuronal progenitor cells and postmitotic neurons in the developing cerebral cortex. DISC1 can interact with both centrosomal proteins and dynein-motor related proteins. In addition, DISC1 interacts with and inhibits the kinase GSK-3β to enable Wnt activation of β-catenin–mediated gene transcription and neuron proliferation. These functions of DISC1 may be regulated by post-translational modification. PKA and CDK5 can phosphorylate Ser-710, and a non-phosphorylatable Ser-710 mutant shows decreased interaction with the centrosomal proteins, BBS1 and BBS4, while a constituitively phosphorylated Ser-710 mutant shows increased interaction with these proteins. During neuronal progenitor cell proliferation, DISC1 phosphorylation at Ser-710 is low and the interaction with GSK-3β is enhanced. By contrast, DISC1 phosphorylation is increased, association with GSK-3β is decreased, and interaction with BBS1 is enhanced during postmitotic neuron migration.
Application Dilution
WB	1:300-5000