| Overview |
| bs-70434r-100ul |
| CrkL (C-terminus) Antibody |
| WB |
| The antibody detects a 38 kDa* protein corresponding to the molecular mass of CrkL on SDS-PAGE immunoblots of human K562 cells, mouse brain tissue, and NGF-differentiated rat PC12 cells. This peptide sequence is well conserved in mouse and rat CrkL, and has less than 50% homology to human Crk II. |
| Human, Mouse, Rat, Chicken, Xenopus |
| Specifications |
| Unconjugated |
| Rabbit |
| CrkL synthetic peptide (coupled to KLH) containing amino acid in the C-terminus of human CrkL. |
| Polyclonal |
| IgG |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| P46109 |
| v-crk sarcoma virus CT10 oncogene homolog, CRKII, CRKL |
| The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl. |
| Application Dilution |
| WB |
1:300-5000 |
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