Troponin I (cardiac) Ser43 Antibody

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Bovine
Sheep
Zebrafish
Xenopus
Non-Human Primate

Overview
Catalog #	bs-70296R
Product Name	Troponin I (cardiac) Ser43 Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser43. Immunolabeling is greatly decreased with λ-phosphatase treatment.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Bovine, Sheep, Zebrafish, Xenopus, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser43
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	21954
Swiss Prot	P48787
Synonyms	cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody
Background	Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser-43 and Ser-45 of cardiac troponin I plays a substantial role in the PKC mediated depression. (Montgomery et al, 2002).
Application Dilution
WB	1:300-5000