PAK-1 (Thr84) Antibody

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Bovine
Sheep
Chicken
Guinea Pig
Zebrafish
Non-Human Primate

Overview
Catalog #	bs-70212R
Product Name	PAK-1 (Thr84) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~68 kDa PAK1 phosphorylated at Thr84. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Bovine, Sheep, Chicken, Guinea Pig, Zebrafish, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 of human PAK1, conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Thr84
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	5058
Swiss Prot	Q13153
Synonyms	ADRB2 antibody, Alpha PAK antibody, Alpha-PAK antibody, MGC130000 antibody, MGC130001 antibody, p21 activated kinase 1 antibody, p21 protein (Cdc42/Rac) activated kinase 1 antibody, p21-activated kinase 1 antibody, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog yeast) antibody, p65 PAK antibody, p65-PAK antibody, P68-PAK antibody, PAK alpha antibody, PAK-1 antibody, Pak1 antibody, PAK1_HUMAN antibody, Paka antibody, PAKalpha antibody, Protein kinase MUK2 antibody, Rac/p21-activated kinase antibody, Serine/threonine-protein kinase PAK 1 antibody, STE20 homolog yeast antibody
Background	PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at Thr-84 and bind to gelsolin (Chen et al, 2004).
Application Dilution
WB	1:300-5000