NF-kB p65 (Ser316) Antibody

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Bovine
Guinea Pig
Goat

Overview
Catalog #	bs-70175R
Product Name	NF-kB p65 (Ser316) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~65 kDa NF-kB p65 protein phosphorylated at Ser316. Immunolabeling is eliminated with λ-phosphatase treatment.
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Bovine, Guinea Pig, Goat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser316 of mouse NF-kB p65, conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser316
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	19697
Swiss Prot	Q04207
Synonyms	Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody, MGC131774 antibody, NF kappa B p65delta3 antibody, NFKB3 antibody, Nuclear Factor NF Kappa B p65 Subunit antibody, Nuclear factor NF-kappa-B p65 subunit antibody, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody, OTTHUMP00000233473 antibody, OTTHUMP00000233474 antibody, OTTHUMP00000233475 antibody, OTTHUMP00000233476 antibody, OTTHUMP00000233900 antibody, p65 antibody, p65 NF kappaB antibody, p65 NFkB antibody, relA antibody, TF65_HUMAN antibody, Transcription factor p65 antibody, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody, V rel avian reticuloendotheliosis viral oncogene homolog A antibody, v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody, V rel reticuloendotheliosis viral oncogene homolog A nuclear factor of kappa light polypeptide gene enhancer in B cells 3 p65 antibody
Background	p65, also known as RelA, is one of the five members of the NF-kB family. The p65 and p50 heterodimer is the predominant form of NF-kB, where p65 is the major subunit (Gilmore et al, 2006). There have been 13 identified phosphorylation sites on p65, including Ser-316 which is induced by IL-1β (Viatour et al, 2005 and Wang et al, 2015). Phosphorylation of Ser-316 is essential for NF-kB nuclear transportation, NF-kB-dependent gene regulation, cytokine/chemokine secretion, and other biological functions (Wang et al, 2015). Ser-316 has also been demonstrated to co-phosphorylate with either Ser-529 or Ser-536 to regulate the majority of NF-kB targeted genes while having its own unique regulation function (Wang et al, 2015). CKI kinase specifically targets phosphorylation at Ser-316 on p65 (Wang et al, 2015). CKI is involved in regulation of membrane transport, cell division, DNA repair, and nuclear localization by phosphorylating its target proteins (Price et al, 2006).
Application Dilution
WB	1:300-5000