MARCKS Antibody

Applications

Reactivity

Human

Predicted Reactivity

Non-Human Primate

Overview
Catalog #	bs-70144CH
Product Name	MARCKS Antibody
Applications	WB, IHC, ICC
Specificity	Specific for endogenous levels of the ~80 kDa MARCKS protein.
Reactivity	Human
Predicted Reactivity	Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Chicken
Source	Recombinant human MARCKS expressed in and purified from E. coli
Clonality	Polyclonal
Isotype	IgY
Concentration	Lot Dependent
Purification	Total IgY fraction
Storage Buffer	PBS + 10 mM NaN3.
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Target
Gene ID	4082
Swiss Prot	P29966
Synonyms	80 kDa protein antibody, 80K L antibody, 80K L protein antibody, 80K-L protein antibody, 80KL antibody,81 kDa protein, light chain antibody, light chain antibody, MACS antibody, MARCKS antibody, MARCS antibody, MARCS_HUMAN antibody, MGC52672 antibody, myristoylated alanine rich C kinase substrate antibody, Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody, Myristoylated alanine rich protein kinase C substrate antibody, Myristoylated alanine-rich C-kinase substrate antibody, Phosphomyristin antibody, PKCSL antibody, PRKCSL antibody, protein kinase C substrate 80 kDa protein light chain antibody, Protein kinase C substrate antibody
Background	Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).
Application Dilution
WB	1:300-5000
IHC
ICC	1:100-500