GluR2-subunit (Ser880) Antibody

Applications

Reactivity

Predicted Reactivity

Human
Mouse
Chicken
Zebrafish
Non-Human Primate

Overview
Catalog #	bs-70125r-100ul
Product Name	GluR2-subunit (Ser880) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~100 kDa GluR2 protein phosphorylated at Ser880. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Chicken, Zebrafish, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser880 conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Ser880
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	29627
Swiss Prot	P19491
Synonyms	AMPA 2 antibody, AMPA selective glutamate receptor 2 antibody, AMPA-selective glutamate receptor 2 antibody, AMPA2 antibody, GluA2 antibody, GLUR 2 antibody, GLUR B antibody, GluR K2 antibody, GluR-2 antibody, GluR-B antibody, GluR-K2 antibody, GLUR2 antibody, GLURB antibody, Glutamate receptor 2 antibody, Glutamate receptor ionotropic AMPA 2 antibody, Glutamate receptor ionotropic antibody, Gria2 antibody, GRIA2_HUMAN antibody, HBGR2 antibody
Background	The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The number of GluR2 subunits in the AMPA receptor complex affects the Ca2+ permeability, rectification and single-channel conductance of AMPA receptors. Ser-880 has been identified as the PKC phosphorylation site within the C-terminal region of GluR2 and has been shown to differentially regulate the interaction of the PDZ domain-containing proteins GRIP1 and PICK 1 (Matsuda et al., 1999)
Application Dilution
WB	1:300-5000