ERK/MAPK (Thr202/Tyr204) Antibody

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

Overview
Catalog #	bs-70084r-100ul
Product Name	ERK/MAPK (Thr202/Tyr204) Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~42 - 44 kDa ERK/MAPK protein phosphorylated at Thr202 and Tyr204. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Bovine, Chicken, Zebrafish, Xenopus, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat ERK/MAPK conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Thr202, Tyr204
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	116590
Swiss Prot	P63086
Synonyms	ERK 1 antibody, ERK 2 antibody, ERK-2 antibody, ERK1 antibody, erk1/2 antibody, ERK2 antibody, ERT1 antibody, ERT2 antibody, Extracellular signal regulated kinase 1 antibody, Extracellular signal-regulated kinase 2 antibody, MAP kinase 1 antibody, MAP kinase 2 antibody, MAP kinase isoform p42 antibody, MAP kinase isoform p44 antibody, MAPK 1 antibody, MAPK 2 antibody, MAPK 3 antibody, Mapk1 antibody, MAPK2 antibody, MAPK3 antibody, Mitogen-activated protein kinase 1 antibody, Mitogen-activated protein kinase 2 antibody, MK01_HUMAN antibody, p38 antibody, p40 antibody, p41 antibody, p42-MAPK antibody, PRKM 2 antibody
Background	Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
Application Dilution
WB	1:300-5000
IHC