| Overview |
| bs-70041r-100ul |
| Che-1 (AATF) (Ser477) Antibody |
| WB |
| Specific for endogenous levels of the ~66 kDa Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Human |
| Mouse, Rat, Dog, Bovine, Sheep, Xenopus, Non-Human Primate |
| Specifications |
| Unconjugated |
| Rabbit |
| Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1, conjugated to keyhole limpet hemocyanin (KLH). |
| Ser477 |
| Polyclonal |
| IgG |
| Lot Dependent |
| Antigen Affinity purification from Pooled whole antiserum |
| 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| 26574 |
| Q9NY61 |
| AATF antibody, AATF_HUMAN antibody, Apoptosis antagonizing transcription factor antibody, Apoptosis-antagonizing transcription factor antibody, BFR2 antibody, CHE 1 antibody, CHE1 antibody, DED antibody, Protein AATF antibody, Rb binding protein Che 1 antibody, Rb-binding protein Che-1 antibody |
| Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006). |
| Application Dilution |
| WB |
1:300-5000 |
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