14-3-3 Protein Antibody

Applications

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

Overview
Catalog #	bs-70000r-100ul
Product Name	14-3-3 Protein Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~29 kDa 14-3-3 protein. Immunolabeling is blocked by preadsorption of the antibody with the peptide immunogen.
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Bovine, Chicken, Zebrafish, Xenopus, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Peptide corresponding to amino acid residues from the N-terminal region of the rat 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	56011
Swiss Prot	P35213
Synonyms	14 3 3 antibody, 14 3 3 protein beta antibody, 14 3 3 protein beta/alpha antibody, 14 3 3 protein zeta antibody ,14 3 3 zeta antibody,14-3-3 protein beta/alpha antibody, 14-3-3 protein/cytosolic phospholipase A2 antibody, 1433B_HUMAN antibody, GW128 antibody, HS1 antibody, KCIP 1 antibody, KCIP-1 antibody, MGC111427 antibody, MGC126532 antibody, MGC138156 antibody, N-terminally processed antibody, Protein 1054 antibody, Protein kinase C inhibitor protein 1 antibody, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein delta polypeptide antibody, Tyrosine 3/tryptophan 5 -monooxygenase activation protein zeta polypeptide antibody, YWHAB antibody, YWHAD antibody, YWHAZ antibody
Background	14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.
Application Dilution
WB	1:300-5000