Phospholipase C beta 1 Antibody

$Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti PLC \u03b21\/Phospholipase C beta 1 Polyclonal Antibody, Unconjugated (bs-6976R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti PLC \u03b21\/Phospholipase C beta 1 Polyclonal Antibody, Unconjugated (bs-6976R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

U2OS cell lysates probed with Phospholipase C beta 1 Polyclonal Antibody, Unconjugated (bs-6976R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Horse
Chicken

Overview
Catalog #	bs-6976R
Product Name	Phospholipase C beta 1 Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Phospholipase C beta 1
Immunogen Range	441-540/1216
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23236
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	1-phosphatidylinositol-45-bisphosphate phosphodiesterase beta 1; 1vphosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 ; Phosphb; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-beta 1; Phospholipase C beta 1 phosphoinositide-specic; Phospholipase C I; PI PLC; PLC 1; PLC_1; PLC-154; PLC-beta-1; PLC-I; PLC154; Plcb; PLCB1; Plcb1 protein; PLCbeta1.
Background	Phospholipase C beta 1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. Its gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for its gene.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200