Catalase Polyclonal Antibody

L1 rat kidney lysates L2 rat brain lysates probed with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 58kD. Observed band size: 58kD

Mouse liver lysates probed with Rabbit Anti-Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

HL-60 cells were fixed with 4% PFA for 10min at room temperature,20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Catalase Polyclonal Antibody (bs-6874R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Placenta tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human HepG2 cell lysates probed with CAT Polyclonal Antibody, Unconjugated (bs-6874) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.