PTGER1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-PTGER1 Polyclonal Antibody, Unconjugated (bs-6316R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-PTGER1 Polyclonal Antibody, Unconjugated (bs-6316R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

Lane 1: mouse kidney lysates probed with Anti PTGER1 Polyclonal Antibody, Unconjugated (bs-6316R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD

$Blank control (Black line): Mouse blood (Black).\r\nPrimary Antibody (green line): PTGER1 Polyclonal Antibody (bs-6316R)\r\nDilution: 3\u03bcg \/10^6 cells;\r\nIsotype Control Antibody (orange line): Rabbit IgG .\r\nSecondary Antibody (white blue line): Goat anti-rabbit IgG\r\nDilution: 1\u03bcg \/test.\r\nProtocol\r\nThe cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.$

Blank control (Black line): Mouse blood (Black).\r\nPrimary Antibody (green line): PTGER1 Polyclonal Antibody (bs-6316R)\r\nDilution: 3\u03bcg \/10^6 cells;\r\nIsotype Control Antibody (orange line): Rabbit IgG .\r\nSecondary Antibody (white blue line): Goat anti-rabbit IgG\r\nDilution: 1\u03bcg \/test.\r\nProtocol\r\nThe cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog

Overview
Catalog #	bs-6316R
Product Name	PTGER1 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Prostaglandin E Receptor EP1
Immunogen Range	61-160/402
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5731
Swiss Prot	P34995
Subcellular location	Cell membrane
Synonyms	EP1; Prostaglandin E2 receptor EP1 subtype; PGE receptor EP1 subtype; PGE2 receptor EP1 subtype; Prostanoid EP1 receptor; PTGER1
Background	Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function. Implicated the smooth muscle contractile response to PGE2 in various tissues.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200