ADORA2B Polyclonal Antibody

RSC96 cells probed with Rabbit Anti-ADORA2B Polyclonal Antibody, PE Conjugated (bs-5900R-PE) at 1:30 for 40 minutes (green) compared to control cells (blue).

Mouse brain lysates probed with Rabbit Anti-ADORA2B Polyclonal Antibody (bs-5900R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.

$HepG2 cells were stained with ADORA2B Polyclonal Antibody, Unconjugated bs-5900R at 1:100 in PBS and incubated for two hours at 37\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

HepG2 cells were stained with ADORA2B Polyclonal Antibody, Unconjugated bs-5900R at 1:100 in PBS and incubated for two hours at 37\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Pig
Rabbit
Guinea Pig

Overview
Catalog #	bs-5900R
Product Name	ADORA2B Polyclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Pig, Rabbit, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ADORA2B
Immunogen Range	101-200/332
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	136
Swiss Prot	P29275
Subcellular location	Cell membrane
Synonyms	ADORA2; Adenosine receptor A2b; ADORA2B
Background	Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500