ADAM12 Polyclonal Antibody

Mouse heart lysates probed with ADAM12 Polyclonal Antibody, Unconjugated (bs-5847R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$U-87MG cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM12 Antibody\uff08bs-5847R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).$

U-87MG cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM12 Antibody\uff08bs-5847R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Chicken
Rabbit

Overview
Catalog #	bs-5847R
Product Name	ADAM12 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ADAM12
Immunogen Range	201-300/909
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8038
Subcellular location	Secreted, Cell membrane
Synonyms	A disintegrin and metalloproteinase domain 12; A disintegrin and metalloproteinase domain 12; ADA12_HUMAN; ADAM 12; ADAM metallopeptidase domain 12; ADAM12; Disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; MCMPMltna; Meltrin alpha; Meltrin-alpha; MLTN; MLTNA; OTTHUMP00000046766.
Background	Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400