Robo1/2 Polyclonal Antibody

$Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; \\nAntigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum 5%) at 37\u2103 for 20 min; \\nIncubation: Anti-Robo Polyclonal Antibody, Unconjugated (bs-5794R) 1:200, overnight at 4\u00b0C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml,blue) was used to stain the cell nuclei.$

Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; \\nAntigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum 5%) at 37\u2103 for 20 min; \\nIncubation: Anti-Robo Polyclonal Antibody, Unconjugated (bs-5794R) 1:200, overnight at 4\u00b0C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml,blue) was used to stain the cell nuclei.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Rat
Chicken

Predicted Reactivity

Human
Mouse

Overview
Catalog #	bs-5794R
Product Name	Robo1/2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Rat, Chicken
Predicted Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Robo1/2.
Immunogen Range	51-150/1651
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6091, 6092
Subcellular location	Cell membrane
Synonyms	Axon guidance receptor; Deleted in U twenty twenty; DUTT 1; DUTT1; FLJ21882; H Robo 1; H-Robo-1; hRobo 1; Robo 1; Robo1; ROBO1_HUMAN; Roundabout 1; Roundabout axon guidance receptor homolog 1; Roundabout homolog 1; Roundabout homolog1 precurser; Roundabout1; SAX 3; SAX3; Robo 2; Roundabout 2; Roundabout axon guidance receptor homolog 2.
Background	Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior (1). In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively (1). These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway (1). One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals (2,3). Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion (1-3). Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross (1). Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout (2-4). Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray (5,6).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200