PRKAR2A Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: PRKAR2A knockout (KO) HeLa cell lysates probed with PRKAR2A Polyclonal Antibody, Unconjugated (bs-55176R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates; Lane 3: SW480 cell lysates; Lane 4: LO2 cell lysates; Lane 5: Mouse Lung lysates; Lane 6: Rat skeletal muscle lysates probed with PRKAR2A Polyclonal Antibody, Unconjugated (bs-55176R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55176R
Product Name	PRKAR2A Polyclonal Antibody
Applications	WB, ELISA
Specificity	KO-Validated
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PRKAR2A (NP_004148.1).
Immunogen Range	1-404/404
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5576
Swiss Prot	P13861
Subcellular location	Cytoplasm, Cell membrane
Synonyms	PRKAR2A;PKR2;PRKAR2
Background	cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000