PARK7 Polyclonal Antibody

$Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Human Jurkat cell lysates probed with PARK7 Polyclonal Antibody, Unconjugated (bs-55163R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Human Jurkat cell lysates probed with PARK7 Polyclonal Antibody, Unconjugated (bs-55163R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: 293T cell lysates; Lane 2: PARK7 knockout (KO) 293T cell lysates probed with PARK7 Polyclonal Antibody, Unconjugated (bs-55163R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PARK7 ) polyclonal Antibody, Unconjugated (bs-55163R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc$

Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PARK7 ) polyclonal Antibody, Unconjugated (bs-55163R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc

Applications

WB
IF(ICC)
IF

Reactivity

Human
Mouse

Overview
Catalog #	bs-55163R
Product Name	PARK7 Polyclonal Antibody
Applications	WB, IF(ICC), IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	11315
Swiss Prot	Q99497
Subcellular location	Cytoplasm, Nucleus
Synonyms	DJ-1;DJ1;GATD2;HEL-S-67p;PARK7
Background	The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IF