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GSS Polyclonal Antibody

Lane 1: DU145 cell lysates; Lane 2: K562 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Brain lysates; Lane 7: Rat Kidney lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: DU145 cell lysates; Lane 2: K562 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Brain lysates; Lane 7: Rat Kidney lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: GSS knockout (KO) 293T cell lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: GSS knockout (KO) 293T cell lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-55091R
Product Name GSS Polyclonal Antibody
Applications WB, IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 2937
Swiss Prot P48637
Subcellular location Cytoplasm
Synonyms GSS;GSHS;HEL-S-64p;HEL-S-88n
Background Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400

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