ERK2 Polyclonal Antibody

Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Mouse Brain lysates; Lane 4: Mouse Spleen lysates; Lane 5: Rat Brain lysates; Lane 6: Rat Spinal node lysates probed with ERK2 Polyclonal Antibody, Unconjugated (bs-55069R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: ERK2 knockout (KO) HeLa cell lysates probed with ERK2 Polyclonal Antibody, Unconjugated (bs-55069R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55069R
Product Name	ERK2 Polyclonal Antibody
Applications	WB, ELISA
Specificity	KO-Validated
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_620407.1).
Immunogen Range	200-300/360
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5594
Swiss Prot	P28482
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	ERK;ERK-2;ERK2;ERT1;MAPK2;P42MAPK;PRKM1;PRKM2;p38;p40;p41;p41mapk;p42-MAPK;MAPK1
Background	This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000