CCND3 Polyclonal Antibody

Lane 1: Human Hela cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Mouse Spleen lysates probed with CCND3 Polyclonal Antibody, Unconjugated (bs-55038R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: CCND3 knockout (KO) HeLa cell lysates probed with CCND3 Polyclonal Antibody, Unconjugated (bs-55038R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bs-55038R
Product Name	CCND3 Polyclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 201-292 of human CCND3 (NP_001751.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	896
Swiss Prot	P30281
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	CCND3;cyclin D3;Cyclin D3
Background	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.
Application Dilution
WB	1:300-5000