Caspase-3 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:100 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:100 overnight at 4\u00b0C, DAB staining.

$Lane 1: Jurkat cell lysates; Lane 2: Jurkat cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n$

Lane 1: Jurkat cell lysates; Lane 2: Jurkat cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n

Lane 1: 293T cell lysates; Lane 2: Caspase-3 knockout (KO) 293T cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human HL-60 cell lysates; Lane 2: Human MOLT4 cell lysates; Lane 3: Human K562 cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HL-60 cell lysates; Lane 2: Human MOLT4 cell lysates; Lane 3: Human K562 cell lysates probed with Caspase-3 Polyclonal Antibody, Unconjugated (bs-55032R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF

Reactivity

Human
Rat

Predicted Reactivity

Mouse

Overview
Catalog #	bs-55032R
Product Name	Caspase-3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF
Reactivity	Human, Rat
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2).
Immunogen Range	55-160/277
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	836
Swiss Prot	P42574
Subcellular location	Cytoplasm
Synonyms	CPP32;CPP32B;SCA-1;Active Caspase 3;CASP3;active Caspase-3;Caspase 3;Caspase-3 p12;caspase-3
Background	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF