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ASK1 (Ser83) Polyclonal Antibody, PE-Cy7 Conjugated

Applications

  • WB

Predicted Reactivity

  • Human
Overview
Catalog # bs-5436R-PE-Cy7
Product Name ASK1 (Ser83) Polyclonal Antibody, PE-Cy7 Conjugated
Applications WB
Predicted Reactivity Human
Specifications
Conjugation PE-Cy7
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Ser83
Modification Site Ser83
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4217
Subcellular location Cytoplasm
Synonyms ASK1 phospho S83; p-ASK1 phospho S83; MAP3K5phospho S83; ASK1Ser83; Mitogen-activated protein kinase kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Apoptosis signal-regulating kinase 1; ASK-1; MAP3K5; ASK1; MAPKKK5; MEKK5; M3K5_HUMAN.
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Application Dilution
WB 1:300-5000