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p53 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IF(ICC)
  • ChIP-seq

Reactivity

  • Human
Overview
Catalog # bs-53127R
Product Name p53 Polyclonal Antibody
Applications WB, ELISA, IF(ICC), ChIP-seq
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein
Clonality Polyclonal
Isotype Not Determined
Concentration 2.5ug/ul
Purification Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Storage Buffer PBS with 0.05% sodium azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 7157
Swiss Prot P04637
Synonyms TP53, P53, TRP53, LSF1
Background Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IF(ICC) 1:50-200
ChIP-seq