MYH11 Polyclonal Antibody

To determine the titer of the antibody, an ELISA was performed using a serial dilution MYH11 Polyclonal Antibody (bs-53091R). The plates were coated with the peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.

$ChIP assays were performed using ME-1 cells, MYH11 Polyclonal Antibody (bs-53091R) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 \u03bcl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. The figure shows the relative occupancy, calculated as the ratio and control\/background for which the MYOG gene was used.$

ChIP assays were performed using ME-1 cells, MYH11 Polyclonal Antibody (bs-53091R) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 \u03bcl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. The figure shows the relative occupancy, calculated as the ratio and control\/background for which the MYOG gene was used.

$The IP\u2019d DNA from 6 ChIP\u2019s was pooled and subsequently analyzed on an Illumina HiSeq 2000. Library preparation, cluster generation, and sequencing were performed according to the manufacturer\u2019s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.$

The IP\u2019d DNA from 6 ChIP\u2019s was pooled and subsequently analyzed on an Illumina HiSeq 2000. Library preparation, cluster generation, and sequencing were performed according to the manufacturer\u2019s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.

Applications

WB
ELISA
ChIP-seq

Reactivity

Human

Overview
Catalog #	bs-53091R
Product Name	MYH11 Polyclonal Antibody
Applications	WB, ELISA, ChIP-seq
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Polyclonal antibody raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein
Clonality	Polyclonal
Isotype	Not Determined
Concentration	Not Determined
Purification	Whole antiserum from rabbit containing 0.05% azide
Storage Buffer	Serum with 0.05% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4629
Swiss Prot	P35749
Synonyms	SMMHC, AAT4, FAA4, SMHC
Background	The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16[inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. MYH11 is a smooth muscle myosin belonging to the myosin heavy chain family. It is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
ChIP-seq